E. coli Uracil-DNA Glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or less bases).

Concentration: 10-20 u/μl

Storage buffer:

• 20 mM Tris-HCl

• 50 mM NaCl, pH 8.0

• 0.1 mM EDTA

• 50% glycerol

Unit definition: One unit of activity is the amount of enzymes required to release 60 pmol of uracil per minute from double-stranded uracil-containing DNA

Reaction buffer (10x):

• 200 mM TrisHCl, pH 8.0

• 10 mM EDTA

• 10 mM DTT

The enzyme is active in water or TE buffer

Storage conditions: -20°C