Enzyme catalyzes the hydrolysis of inorganic pyrophosphatase to form orthophosphatase:

P₂O₇^-4 + H₂O → 2HPO₄^-2.

Enzyme is extremely thermostable.

Source: Isolated from E. coli strain that carries the cloned inorganic pyrophosphatase gene from Thermococcus litoralis

Concentration: 1000 units/ml

Unit Definition: One unit is the amount of enzyme that will generate 40 nmoles of phosphate per minute from pyrophosphate under standard reaction conditions (50 mM Tris-HCl, pH 8.5; 1 mM MgCl 2 , 0.32 mM PPi) at 75°C

Reaction Buffer (x10):

• 500 mM Tris-HCL (pH 8.5)

• 10 mM MgCl₂

Storage buffer:

• 20 mM Tris-HCL (pH 7.6)

• 100 mM KCl

• 0.1 mM EDTA

• 0.2% Tween-20

• 50 % glycerol

Storage conditions: -20 °C