SuperHotTaq (Hot Start) DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22°C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with SuperHotTaq are free from unspecific products and from primer-dimers.

Applications:

•Hot Start PCR

•Real Time PCR

•Amplification of complex genomic and cDNA templates

•Multiplex PCR

•High specifity PCR

Storage Conditions:

-20°C

Concentration:

5 units/µl (or high concentrated 17.5 u/µl)

Storage buffer:

10 mM Tris-HCI (pH 7.0)

50 mM KCl

0.1 mM EDTA

50% glycerol

Recommended PCR conditions:

Use PCR conditions optimized for Taq DNA Polymerase. In the case of a low amount of DNA template, additional cycles should be used.

Unit definition:

One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72°C.

Supplied Reaction buffers (10x):

Complete NH 4 Reaction buffer - Ammonium buffer with 25 mM MgCl 2

Incomplete NH 4 Reaction buffer - Ammonium buffer without MgCl 2

Complete KCl Reaction buffer - Potassium buffer 15 mM MgCl 2

Included MgCl 2 (100 mM)

We recommend Ammonium buffer for increased yield of PCR products and potassium buffer for increased specificity of PCR.

MgCl 2 concentration should be optimized for each particular primer-template combination, meanwhile PCR is effective with 2.5 mM of MgCl 2 in the majority of cases.

Recommended Reaction Buffer (x1):

16 mM (NH4)2SO4

67 mM Tris-HCl (pH 8.8)

1.5 – 7 mM MgCl 2

0.01% Tween-20

•Reliable and reproducable quantification in qPCR

•Perfect for Real Time PCR

•Especially for diagnostic purposes

•Reaction set-up at room temperature

•Activation of enzyme during first heating

•No change or optimization of protocol necessary

•High specifity, reduced primer mismatch or dimers

Comparíson Chart:

Recently SuperHotTaq DNA Polymerase and 15 others Thermophilic DNA polymerases from the major suppliers of enzymes for molecular biology were tested for performance and sensitivity in Mycoplasma pneumonia and Mycoplasma genitalium detection tests. The sample contains dilutions of tested DNA and constant amount of positive control D.

SuperHotTaq DNA Polymerase from BIORON was show n to be the best in comparison with all competitor's enzymes.

from left to right: 1. 100bp DNA ladder 2. Negative control 3. M. genitalium DNA, no dilution 4. M. genitalium DNA 10-1 dilution 5. M.genitalium DNA 10-2 dilution 6. M. genitalium DNA 10-3 dilution 7. M. genitalium DNA 10-4 dilution