Purified from E. coli WA921/pST27 hsd+.

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5’…C    CWWGG…3’

3’…GGWWC    C…5’

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Concentration: 8-12 u/μl

Ligation/recutting assay: After 50-fold overdigestion with the enzyme, more than 98% of the DNA fargments can be ligated and recut

Nonspecific endonucleases test: 16 hours incubation of 1 μg of DNA with 50 units of the enzyme at 37°C results in no unspecific cleavage product

Reaction conditions:

• 100 mM NaCl

• 50 mM Tris-HCl (pH 7.9 at 25°C)

• 10 mM MgCl 2

• 1 mM dithiothreitol

• 100 μg/ml BSA

Incubate at 37°C

Storage buffer:

• 50 mM KCl

• 10 mM Tris-HCl (pH 7.4)

• 0.1 mM EDTA

• 1 mM dithiothreitol

• 200 μg/ml BSA

• 50% glycerol

Storage conditions: -20°C

Absence of contaminants: 50 units of Sty I do not produce any unspecific cleavage products after 16 hours incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with Sty I, more than 98% of the DNA fragments can be ligated and recut with this enzyme

Heat inactivation: 65°C for 20 minutes