Fragment sizes (Base Pairs):

10.0, 8.0, 6.0, 5.0, 4.0, 3.0×2, 2.5, 2.0, 1.5, 1.0×2, 0.75, 0.5, 0.25.

1 kb ready-to-use DNA Ladder is ideal for determining the size of double-stranded DNA from 250 to 10,000 base pairs. The ladder consists of 13 fragments. Ten fragments are the products of the restrictions of special plasmids by EcoRV and/or RsaI restrictases. 750, 500 and 250 bp fragments are PCR products. Pfu DNA polymerase was used for amplification. The 3.0 kb fragment and 1.0 kb fragment are present at increased intensity to allow easy identification. All fragments are blunt-ended. Recommended volume for loading is 5-6 µl per lane.

Storage buffer:

10 mM Tris-HCI, pH 7.5

1 mM EDTA

10 mM NaCl

6% glycerol

0.05% Xylene Cyanol

Storage Conditions:

-20°C